A, B. The cell proliferation of the indicated stable cell lines in vitro was measured at different time points, as indicated by the CCK-8 assay. The bars correspond to the mean± standard error, and the P value was calculated using Student's t-test. *P<0.05, **P<0.01. C, D. The colony formation of the indicated stable cell lines in vitro was measured for 14 days, as described in the Methods. The bars correspond to the mean± standard error, and the p-value was calculated using Student's t-test. **P<0.01,***P<0.001. E, F. The stable cell lines silencing the negative control or TRIM11 were treated with 150 μg/ml5-FU for 24 h and then subjected to annexin V-FITC and PI staining. Cell apoptosis was evaluated through FACS. The bars correspond to the mean± standard error (n = 3), and the p-value was calculated using Student's t-test. *P<0.05.