Figure 6. Cholestasis in Ctnnb1TCCA hep mice is not a consequence of developmental abnormalities.

Tamoxifen injection scheme for SA-CreER^TTom and Ctnnb1^{TCCA hep} mice (A). Tamoxifen administration leads to the activation of the Cre recombinase and consequently to expression of tdTomato (red fluorescence) in hepatocytes but not in cholangiocytes (counterstaining with DAPI, bd = bile duct, pv = portal vein) (B). Serum ALT, AST and bile acid levels were significantly increased in Ctnnb1^{TCCA hep} mice (C and D). Ctnnb1^{TCCA hep} mice display only minor signs of fibrosis (E and F).