Figure 7. Ctnnb1TCCA hep mice display deregulation of key enzymes for bile acid synthesis and hepatic transport.

Expression of Cyp7a1 (A) was increased in Ctnnb1^{TCCA hep} mice, whereas mRNA levels of Cyp27 (B), Cyp8b1 (C), Fgfr4 (D) and Fxr (E) were significantly reduced. mRNA levels of Abcb11 were reduced (F) and of Abcc2 increased in Ctnnb1^{TCCA hep} mice (G). Downregulation of Ntcp (H) and upregulation of Abcc4 (I) in livers with continuous β-catenin activation. Deregulation of bile acid transporters was confirmed at protein level by Western blot analysis with indicated antibodies (J).