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. 2016 Nov 23;7(52):87100–87113. doi: 10.18632/oncotarget.13527

Figure 3. Knockdown KIAA0247 promoted proliferation of glioma cells.

Figure 3

(A) qRT- PCR was conducted to determine the mRNA levels of KIAA0247 in tin U87 and SHG44 cells. (B, C) Expression of KIAA0247 in U87 and SHG44 cells was analyzed by western blot. (D, E) Cell proliferation was detected in U87 and SHG44 cells by CCK-8 assay. (F–H) Cell cycle distributions were tested in in U87 and SHG44 cells by flow cytometry. NC: blank control cells; shCON: transfected with control shRNA; shKIAA0247 transfected with shKIAA0247 vectors. Data were based on at least 3 independent experiments, and shown as the mean ± SD. (**P < 0.01, ***P < 0.001 compared with NC).