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. 2016 Nov 23;7(52):87147–87160. doi: 10.18632/oncotarget.13515

Figure 4. TAp63 regulates microtubule formation and invasion through miR-133b.

Figure 4

HCT-116 cells were transfected with the miR-133b-expressing vector or negative controls (NCs), or co-transfected with the TAp63-expressing vector and miR-133b sponge (TAp63+sponge). The expression of α-tubulin was analyzed via A. qRT–PCR, B. western blotting and C. immunofluorescence, *P < 0.05, #P>0.05. D. Transwell invasion assays of HCT-116 and SW-620 cells transfected with either TAp63 or NC. After 48 h, invasive cells were counted in five random high-power fields. All of the data represent the means±sd of three different experiments analyzed in triplicate. *P < 0.05, #P>0.05.