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. 2016 Nov 24;7(52):87323–87341. doi: 10.18632/oncotarget.13556

Figure 8. VACO5 colorectal cells still undergo apoptosis when co-depleted for CIT-K and p53.

Figure 8

A. VACO5 cells were treated with control, CIT-K or combination CIT-K/TP53 siRNA for 72 h, where cells were harvested and stained with annexin V and PI to detect apoptosis. 50,000 cells were used in each analysis. B. Quantification of the early apoptotic fraction (annexin V positive, PI negative). Error bars represent SEM, n=4, *p < 0.05 (Student's t-test), n.s., not significant. C. VACO5 cells were treated with control, CIT-K or combination CIT-K/TP53 siRNA for 72 h, where cells were harvested and protein extracts analyzed by Western blot to detect CIT-K, p53 and tubulin. The numbers on the left indicate the sizes in kilodaltons of the molecular mass marker.