Skip to main content
. 2016 Nov 29;7(52):87431–87448. doi: 10.18632/oncotarget.13704

Figure 3. Sema3E is overexpressed in human PDAC lines.

Figure 3

A panel of human PDAC cell lines, plus the normal pancreatic ductal cell line, HPDE, was screened for Sema3E protein and gene expression. A. Immunoblot analysis (left panel) show that all of the PDAC cell lines express moderate to high levels of Sema3E protein, while HPDE expressed very minimal levels of the protein. Densitometry analysis of Sema3E protein levels relative to GAPDH protein levels are shown on the right panel. (**p<0.01; ***p<0.001). B. RT-PCR analysis in the same panel of PDAC cell lines confirms low gene expression of Sema3E in HPDE as compared to the cell lines, MiaPaCa-2, Panc-1 and Panc-28. (*p<0.05; **p<0.01). All data are represented as mean ± S.E.M, and are representative of at least 3 independent experiments.