Skip to main content
. 2016 Nov 29;7(52):87431–87448. doi: 10.18632/oncotarget.13704

Figure 4. Overexpression of Sema3E in PDAC cell lines increases cell growth, proliferation and clonogenic potential, as well as cell migration.

Figure 4

A. MTT assay shows that both Mia-S3E and Panc48-S3E cells had significantly greater cell growth over the period of 6 days compared to their respective controls (two-way ANOVA and Bonferroni posttest, ***p<0.001). B. In a colony formation assay, Mia-S3E cells had a higher rate of colony growth compared to the control, indicating increased clonogenic potential (Student's t-test, ***p<0.001). C. Cell cycle analysis shows an increased rate in cell proliferation in Mia-S3E cells compared to the control, as indicated by a significant G1 to S phase shift (Student's t-test, **p<0.01). D. A wound healing scratch assay shows that Panc48-S3E cells had increased cell movement towards the center of the wound scratch compared to the vector control over 72 h (two-way ANOVA and Bonferroni posttest,**p<0.01). Red squares are small sections in the wound area that got further magnified to demonstrate more wound closure in Panc48-S3E cells vs. the control cells Panc48-VCtrl. E. In a transwell migration assay using uncoated transwells, Sema3E-overexpressing Panc-48 cells similarly had increased cell migration compared to the control (Student's t-test, *p<0.05). F. In a transwell invasion assay using transwells coated with matrigel, Sema3E-overexpressing Panc-48 cells had increased cell invasion compared to the control (Student's t-test, *p<0.05). All data are represented as mean ± S.E.M, and are representative of at least 3 independent experiments.