Fig. 4.
Widely separated repeats are detected by RIP in the absence of Spo11 and MEI-3. Each parental strain carries a pair hygromycin-resistance cassettes (hph) replacing spo11 and mei-3 genes. All parental hph cassettes have the original sequence introduced by the Neurospora Genome Project (Colot et al. 2006). RIP mutation of the hph cassettes replacing the mei-3 gene was assessed in the same progeny spores used previously to analyze mutation of closely positioned repeats (Gladyshev and Kleckner 2014)