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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Neurosci Biobehav Rev. 2016 Sep 14;76(Pt B):380–395. doi: 10.1016/j.neubiorev.2016.09.007

Figure 2.

Figure 2

Amygdalar FAAH activity in male and female mice at different ages. Twenty-four hours after EPM, mice were decapitated; brains were removed and frozen on dry-ice. The amygdala was dissected on dry-ice, weighed and membranes were harvested as described previously (Hillard et al., 1995). Membranes were incubated with 0.2 nM [3H]AEA for 10 min. FAAH activity was calculated as the ratio of dpm (disintegration per minute) in the aqueous phase and the total dpm (aqueous + organic). *p<0.05 compared to 4–6 week old group.