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. Author manuscript; available in PMC: 2017 Apr 25.
Published in final edited form as: Mucosal Immunol. 2016 Sep 14;10(3):673–684. doi: 10.1038/mi.2016.80

Figure 3.

Figure 3

CXCL1 and CXCL2 are induced by IL-17A and blocking CXCR2 reduces neutrophil influx in response to colonization with SFB-containing microbiota. (a) Expression of CXCL1 and CXCL2 mRNA were examined by qPCR in the ileum of SFB-void Jax mice colonized with SFB+CC over the indicated time course. (b) Expression of CXCL1 and CXCL2 mRNA in the ileum of SFB-void Jax mice (Jax), and SFB-void Jax mice treated with isotype control antibody or αIL-17A antibody and colonized with SFB+CC for 7 days. Data are representative of two independent experiments with four mice per group. (c) Representative FACS plots with associated (d) frequencies and numbers of neutrophils in the ileum of SFB-void Jax mice (Jax), and SFB-void Jax mice treated with isotype control antibody or αCXCR2 antibody and colonized with SFB+CC for 7 days. Data are representative of four to six mice per group. All data are presented as mean ± SEM; **p<0.01 and ***p<0.001, one-way ANOVA with Dunnett's multiple comparison test (a) or one-way ANOVA with Tukey's multiple comparison test (b,d).

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