Figure 4.
Rag-deficient mice colonized with SFB-containing microbiota fail to induce IL-17A, CXCL1, and CXCL2, and display defective neutrophil recruitment into the ileum. Expression of (a) IL-17A and (b) CXCL1 and CXCL2 mRNA were examined by qPCR in the ileum of SFB-void Jax B6 and Rag1−/− mice, and SFB-void Jax B6 and Rag1−/− mice colonized with SFB+CC for 7 days. (c) Representative FACS plots with associated (d) frequencies and numbers of neutrophils isolated from the ileum of SFB-void Jax B6 and Rag1−/− mice, and SFB-void Jax B6 and Rag1−/− mice colonized with SFB+CC for 7 days. Data are representative of two independent experiments with three to four mice per group. All data are presented as mean ± SEM; *p<0.05, **p<0.01, and ***p<0.001, one-way ANOVA with Tukey's multiple comparison test.