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. Author manuscript; available in PMC: 2017 Mar 14.
Published in final edited form as: J Mol Biol. 2016 Dec 6;429(2):237–248. doi: 10.1016/j.jmb.2016.11.030

Figure 3. Gmf1 and Crn1 synergize in inhibiting Arp2/3-mediated actin assembly in vitro.

Figure 3

(A, B) Bulk actin polymerization assays containing G-actin (2 µM, 5% pyrene-labeled) and the indicated concentrations of Arp2/3 complex, VCA, Gmf1, and Crn1. (C) Bulk actin polymerization assays, performed as in A and B, except using full-length Las17 (yeast WASP) instead of VCA. (D) Percentage of Arp2/3 complex particles with Crn1 bound in the presence and absence of Gmf1 (- Gmf1, n = 366; + Gmf1, n = 398).