Figure 3.

Western blot analysis of the activation of the JAK‐STAT pathway in response to SCI. Western blot and histogram analyses showing the levels of phosphorylated STAT3 (pSTAT3) and total STAT3 in the spinal cord after injury and compared with a sham or uncut controls for (A), (B) R stage, (C), (D) NR56 stage and (E), (F) NR66 stage. The presence of STAT3α and STAT3β isoforms is indicated by arrowheads. Western blots are representative of three independent assays for R stage and two assays for each NR stage through 3 hpt and 6 dpt; later times are unique samples. Histograms show the pSTAT3/STAT3β ratio normalized to controls. Control levels (ratio = 1) are indicated by dashed lines. No difference between time points was observed by multiple comparisons with a one‐way ANOVA test.