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. 2017 Mar 15;10:70. doi: 10.3389/fnmol.2017.00070

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Copyright © 2017 Bernal Sierra, Haseleu, Kozlenkov, Bégay and Lewin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EGFP expression during development in spinal cord and DRG of Ca_v3.2^EGFP knock-in mice. (A) At E9.5, EGFP is expressed in some cells of the neuronal crest (nc) indicated by an arrow head, in the epithelium indicated by an arrow, and in the spinal cord. (B) At E13.5, a few cells of the DRG are EGFP⁺, while the staining at the dorsal side of the spinal cord is stronger. (C) At E16.5 the number of EGFP⁺ cells increases in the DRG, but staining in the spinal cord is reduced. (D) Finally at E18.5, EGFP expression in the DRG appears constant while the positive staining has almost disappeared in the spinal cord. Dashed lines outline the DRGs. Scale bars: 200 μm.