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. 2017 Mar 15;8:407. doi: 10.3389/fmicb.2017.00407

FIGURE 2.

FIGURE 2

(A) Phylogenetic tree and (B) sequence alignment of the incRNAI-II of the IncB/O/K-group plasmids including references. The sequences were extracted from the IncK2 plasmids as well as from the reference plasmids of IncB and IncK. The IncB/O incRNAI-II reference sequence was derived from the paper of Couturier et al. (1988). Due to the absence of the complete nucleotide sequence of the IncB/O plasmid, pMU720 was not included into Figure 3. The phylogenetic tree was constructed by UPGMA method and Jukes-Cantor correction [Multiple alignment (open gap penalty (OG): 100%, unit gap penalty (UG): 0%, gap penalty: 100%), 2000 Bootstraps] using Bionumerics 7.6 (Applied Maths, Kortrijk, Belgium). H, human urine sample; P, poultry; PM, poultry meat.