Figure 1.

Live vaccine strain (LVS) fails to replicate in Slc11a1⁺ bone marrow-derived macrophage (BMDM). BMDMs from either B6 (black bars) mice or Slc11a1⁺ (red bars) mice were infected with LVS at a multiplicity of infection of 1 (A) or 100 (B). At the indicated times, macrophages were lysed and bacteria were enumerated by plating serial dilutions on chocolate agar. The solid line represents the limit of detection (LOD). A Student’s t-test on log-transformed data was used to calculate significance. N = 3 wells per time point, data are representative of three experiments of similar design. Error bars indicate SEM.