Figure 3.

Slc11a1⁺mice show no weight loss or detectable bacteria after intranasal live vaccine strain (LVS) inoculation. B6 (black circles) or Slc11a1⁺ (red squares) mice were infected with 1,300 CFU of LVS via the intranasal route. (A) Mice were weighed daily as an indication of disease progression. (B) At 3 days post-infection organs were harvested from five mice and bacterial burdens were enumerated by plating serial dilutions on chocolate agar. The solid line represents the limit of detection (LOD). A Student’s t-test on log-transformed data was used to calculate significance. N = 5 mice per time point, data are representative of three experiments of similar design.