Figure 4.

Fewer bacteria are recovered from Slc11a1⁺ Tlr2/4/9^−/− mice than B6 mice following intranasal live vaccine strain (LVS) inoculation. C57Bl/6 (black circles), Slc11a1⁺ (red squares), or Slc11a1⁺ Tlr2/4/9^−/− (blue triangles) mice were intranasally infected with 1,300 CFU of LVS. At 3 (A) and 5 (B) days post-infection organs were harvested and bacterial burdens were enumerated by plating serial dilutions on chocolate agar. The solid line represents the limit of detection (LOD). A Student’s t-test on log-transformed data corrected for multiple comparisons was used to calculate significance. Unless indicated, differences are not significant. N = 3 mice per time point, data are representative of two experiments of similar design.