Figure 7.

Slc11a1⁺ mice show little detectable changes in lung cytokine after live vaccine strain (LVS) intranasal infection. B6 (black bars), Slc11a1⁺ (red bars), or Slc11a1⁺ Tlr2/4/9^−/− (blue bars) mice were inoculated with 1,300 CFU of LVS via the intranasal route. At the indicated time point, lungs were harvested and processed into a single cellular suspension. After 24 h incubation, supernatants were harvested and cytokines were quantified by cytometric bead array. A Student’s t-test corrected for multiple comparisons was used to calculate significance. Unless indicated, differences are not significant. N = 3 mice per time point, data are representative of two independent experiments of similar design.