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. 2017 Mar 15;8:159. doi: 10.3389/fphys.2017.00159

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Copyright © 2017 Carneiro, Clevelario, Padilha, Silva, Kitoko, Olsen, Capelozzi, Rocco and Cruz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Collagen fibers. Representative photomicrographs (polarized light microscopy) of lung parenchyma and granuloma tissue stained with Picrosirius Red (right panels). Collagen fibers are shown in orange. Original magnification 200 ×. Scale bars = 200 μm. Left panels depict collagen fiber content quantification in lung parenchyma (upper panel, A) and granuloma (lower panel, B). White bar, DMSO; gray bar, BOS. Boxes show the interquartile (25–75%) range, whiskers encompass the range (minimum–maximum), and horizontal lines represent median values of 8 animals per group. ^*Significantly different from C-DMSO (p < 0.05). ^**Significantly different from SIL-DMSO (p < 0.05).