Table 1.
Bovine oligonucleotides used in this study.
| Specificity | Primer | Sequence (5′−3′) | Fragment size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|---|
| IL-1β | Forward | GCAGAAGGGAAGGGAAGAATGTAG | 198 | 52 | Alva-Murillo et al., 2014b |
| Reverse | CAGGCTGGCTTTGAGTGAGTAGAA | ||||
| IL-6 | Forward | AACCACTCCAGCCACAAACACT | 179 | 57 | Alva-Murillo et al., 2014b |
| Reverse | GAATGCCCAGGAACTACCACAA | ||||
| IL-8 | Forward | TTCCACACCTTTCCACCCCAA | 149 | 53.5 | Alva-Murillo et al., 2014b |
| Reverse | GCACAACCTTCTGCACCCACTT | ||||
| IL-10 | Forward | GATGCGAGCACCCTGTCTGA | 129 | 59 | Alva-Murillo et al., 2014b |
| Reverse | GCTGTGCAGTTGGTCCTTCATT | ||||
| RANTES | Forward | CACCCACGTCCAGGAGTATT | 117 | 54 | Nelson et al., 2010 |
| Reverse | CTCGCACCCACTTCTTCTCT | ||||
| GAPDH | Forward | TCAACGGGAAGCTCACTGG | 237 | 56.9 | Yonezawa et al., 2009 |
| Reverse | CCCCAGCATCGAAGGTAGA |