Figure 6.

The Tyr kinase Fyn binds APP in AD neurons: (A) WB analysis performed on total lysate from control (C18) and AD neurons before or after TC2153 (TC) exposure. Samples were probed with Src pTyr₄₁₆ and Src pTyr₅₂₇ antibodies used to analyse Fyn pTyr₄₂₀ and Fyn pTyr₅₃₁ levels and were normalized to the corresponding total Fyn levels and expressed as % of control. β-actin was used as loading control (B). (C) IP analysis of controls (C18 and C16) and AD neurons (LV, ML, and AE). Samples were immunoprecipitated with rabbit anti-pan Fyn antibody and analyzed using rabbit anti-Src pTyr₄₁₆ and rabbit anti- Src pTyr₅₂₇ antibodies (used to detect Fyn pTyr₄₂₀ and pTyr₅₃₁ levels; Xu et al., 2015) or mouse anti-APP (clone Y188). Data were normalized to the corresponding input Fyn levels, and expressed as % of the correspondent C18 values (D). Densitometric analysis of (A,C) is reported in (B,D). Statistically significant differences were calculated by one-way ANOVA and Tukey's post hoc test.