Figure 1.

Macrophage Gal/GalNAc lectin recognizes F. hepatica glycoconjugates and potentiates the production of IL-10 and TNF-α by Pam3CSK4-stimulated monocyte-derived DCs (mo-DCs). (A) Binding of different hCLR-Fc on FhTE-coated plates in presence (white bars) or absence (black bars) of EGTA. (B) Expression of hMGL and DC-SIGN (as differentiation marker) on mo-DCs was confirmed by flow cytometry. (C) IL-6, IL-10 and TNFα levels on supernatants from Pam3CSK4 and LPS-stimulated mo-DC cultures incubated with and without FhTE. (D) moDCs were stimulated as in (B), in the presence of an anti-hMGL antibody or isotype control. A representative result of one out of four donors is shown (±SEM, indicated by error bars). Asterisks indicate statistically significant differences (*p < 0.05).