Figure 3.

Characterization of FhTE glycoconjugates that are recognized by hMGL. (A) hMGL binding was evaluated on FhTE-coated plates with hMGL-Fc previously incubated with EGTA, mannan, GalNAc, anti-hMGL antibody, or isotype control. (B) Western Blot with hMGL-Fc on FhTE. Recognition of FhTE by hMGL was abrogated with EGTA and with periodate oxidation treatment of FhTE. (C) Lectin blot of FhTE using the GalNAc-specific lectin Vicia Villosa (VVL). (D) Inhibition of hMGL binding to plate-bound FhTE with the lectins VVL and ConA (1, 0.1, and 0.1 μg/ml). (E) Inhibition of hMGL binding to plate-bound FhTE with different carbohydrate-specific antibodies. (F) hMGL binding on FhTE-coated plates previously incubated with mannase or GalNAcase. (G) Binding of hMGL on membrane-associated parasite component-coated plates in presence (white bars) or absence (black bars) of EGTA. A representative figure of three or four independent experiments is shown (±SD, indicated by error bars). Asterisks indicate statistically significant differences (*p < 0.05).