Figure 1.

Generation and characterization of CLIC1 null mice. (A) Maps of the WT allele, the targeting vector, and the predicted allele resulting from homologous recombination are shown. Numbered exons are designated by thick bars. N indicates NheI restriction sites and the size of NheI restriction fragments are shown. (B) Southern blot of NheI‐digested genomic DNA from one WT and two heterozygous homologous recombinant ES cell lines, probed with the 3′ probe designated in panel A, showing the 6.2 kB WT fragment and the 9.2 kB recombinant fragment. (C) Western blot of 50 μg total kidney protein from Clic1 (‐/‐) or WT mice as labeled, probed with CLIC1 specific antibody AP1089. Migration positions of molecular weight standards (in kD) are marked. (D) Number of observed offspring of each genotype in 110 consecutive pups from crosses between CLIC1 heterozygotes (dark bars). Error bar represents standard error. The expected Mendelian ratio of 0.25:0.5:0.25 is plotted for comparison (light bars). (E) Growth curves for the 110 pups. Males: filled symbols; Females: open symbols; WT: diamonds; Heterozygotes: squares; KO: triangles. There is no difference in weight among the male offspring. The KO females are significantly smaller than the WT females at all time points, with the Het females having an intermediate average weight.