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. 2017 Mar 8;5(5):e13169. doi: 10.14814/phy2.13169

Figure 5.

Figure 5

Immunofluorescent localization of CLIC1 in kidney. A–C: Low‐power images from renal cortex immunostained for CLIC1 with AP1089 antibody and imaged identically. Scale bar in A represents 400 microns and applies to panels A–C. (A) C1KO control mouse. (B) WT control mouse. The most prominent staining is in the brush border of proximal tubule. Glomeruli are also stained. (C) WT mouse 48 h after folic acid injury. CLIC1 staining is increased and is brightest in proximal tubule. Many dilated tubule segments are visible. (D–G) High‐power images of kidneys from WT mice. Scale bar in D represents 50 microns and applies to panels D–G. (D) WT control kidney showing CLIC1 stain. (E) Same field as in D, showing CLIC1 stain (green) and LTA lectin (red) marking proximal tubule brush border. Notice diffuse CLIC1 staining of brush border and speckled staining of proximal tubule nuclei. (F) WT mouse kidney 48 h after folic acid injury showing CLIC1 stain. (G) Same field as in F, showing CLIC1 stain (green) and LTA lectin (red). Staining has concentrated in the apical pole of the proximal tubule, nuclear staining has largely disappeared.