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. 2016 Nov 30;26(2):263–277. doi: 10.1007/s11248-016-9998-5

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Homology arm length and symmetry influence targeting efficiency. a Diagram illustrating pKOMP-Nxn constructs with various symmetrical homology arm lengths. b Bar graph showing the number of G418-resistant colonies post-electroporation with pKOMP-Nxn with five different homology arm lengths (900, 500, 250, 125, and 0 bps). c Diagram illustrating pKOMP-Nxn constructs with asymmetrical homology arm lengths. d Bar graph showing the number of G418-resistant colonies post-electroporation with pKOMP-Nxn carrying asymmetrical homology arm lengths. F-sgRNA-Nxn = FokI-sgRNA-Nxn