Table 2.
CRISPR/Cas9 protein mediated multi-vector targeting in mouse zygotes
| Targeting vector ID | Cas9 protein (ng/μl) | sgRNA (ng/μl) | Targeting vector (ng/μl) | # Embryos transferred | # Pups | Genotype positive Glt8d1 | Genotype positive Lrrk2 | Genotype positive Gltsd1 and Lrrk2 | % Positive relative to # embryos transferred Glt8d1 | % Positive relative to # embryos transferred Lrrk2 | % Positive relative to # embryos transferred Glt8d1 and Lrrk2 | % Positive relative to # pups Glt8d1 | % Positive relative to # pups Lrrk2 | % Positive relative to # pups Glt8d1 and Lrrk2 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pKOMP-Glt8d1- | ||||||||||||||
| (~900 bp HAs) | 10 + 10 | 5 | ||||||||||||
| & | 50 | & | & | 62 | 9 | 1 | 0 | 1 | 2 | 0 | 2 | 11 | 0 | 11 |
| pKOMP-Lrrk2- | ||||||||||||||
| (~900 bp HAs) | 10 + 10 | 5 |