FIG 4.

The Hvo_0405 Tat signal peptide is critical for ArtA-dependent C-terminal processing. Western blot analysis of proteins isolated from the cytoplasmic (c), membrane (m), and supernatant (s) fractions of the Δhvo_0405 mutant expressing the wild-type C-terminally His-tagged Hvo_0405 (Hvo_0405RR) or C-terminally His-tagged Hvo_0405 with a mutated twin lysine signal sequence (Hvo_0405KK), using either anti-Hvo_0405-N-term antibodies (A) or anti-penta-His antibodies recognizing the C-terminal 6×His tag (B). Numbers indicate molecular masses in kilodaltons, and the arrowhead indicates the unprocessed full-length Hvo_0405. The images shown represent the results from at least three independent replicates tested.