FIG 4.

Cotranscription of Tcr_0853 and Tcr_0854 in wild-type T. crunogena cells cultivated in chemostats under low- and high-DIC conditions. Agarose gel-electrophoresed, ethidium bromide-stained, RT-PCR amplicons are shown for each RT-PCR target, in five lanes, from left to right: genomic DNA, cells cultivated under low-DIC conditions, cells cultivated under low-DIC conditions (reverse transcriptase-free control), cells cultivated under high-DIC conditions, and cells cultivated under high-DIC conditions (reverse transcriptase-free control). Transcription of Tcr_0841 (csoS3) was monitored to verify CCM induction; Tcr_0350 was targeted to verify that the mRNA was of sufficient quality to act as an RT-PCR template.