TABLE 4.

Fold change in Tcr_0853, Tcr_0854, and csoS3 transcript abundance after 2 h of cultivation under low-DIC conditions

Locus tag	Strain	Fold increasea	ΔΔCT ± SDb	Amplicon positionc
Tcr_0853	Wild type	643	−9.3 ± 0.5	1283–1415
	Tcr_0668-1614-WF	1,351	−10.4
	csoS3-399-2XO	1,097	−10.1
	Tcr_0853-710-WF	137	−7.1
	Tcr_0854-737-MF	1,097	−10.1
	Tcr_0854-2006 mutant	4,389	−12.1
Tcr_0854	Wild type	712	−9.5 ± 0.1	1200–1339
	Tcr_0668-1614-WF	832	−9.7
	csoS3-399-2XO	549	−9.1
	Tcr_0853-710-WF	5	−2.3*
	Tcr_0854-737-MF	2	−1.1*
	Tcr_0854-2006 mutant	1,176	−10.2
csoS3	Wild type	2,740	−11.4 ± 0.8	588–736
	Tcr_0668-1614-WF	10,809	−13.4
	csoS3-399-2XO	37	−5.2*
	Tcr_0853-710-WF	6,208	−12.6
	Tcr_0854-737-MF	5,405	−12.4
	Tcr_0854-2006 mutant	14,263	−13.8

^aFold increase in transcript abundance is calculated as low DIC/high DIC (= 2^−ΔΔCT).

^bFor the wild type, standard deviations are based on two cultures that were cultivated under high-DIC conditions before exposure to low-DIC conditions. For the others, a single culture was cultivated (n = 1). Values with asterisks were detected as outliers from the others (P < 0.05) using Grubb's test.

^cPosition, in nucleotides, relative to the first nucleotide of the start codon of the gene.