FadR regulates the expression of genes involved in FA degradation and UFA biosynthesis. The genetic organization of each of the genes is shown. The red boxes indicate the positions of the FadR binding sites (shown in Fig. 4), and the arrows show the putative transcriptional start sites. The positions of the GG mutations are shown by asterisks and dots. From left to right: WL1031, WL1035 (*, P = 0.003), WL1060 (*, P = 0.005), and WL1062 (*, P = 0.005) (A); WL1027, WL1029 (*, P = 0.002), WL1056 (*, P = 0.0001), and WL1058 (*, P = 0.002) (B); WL1040, WL1042 (*, P = 0.005), WL1064 (*, P = 0.01), and WL1066 (*, P = 0.0001) (C); WL1005, WL1007 (*, P = 0.0007), WL1020 (*, P = 0.002), and WL1022 (*, P = 0.002) (D); GK1609, GK1610 (*, P = 0.002), GK1669 (*, P = 0.02), and GK1672 (*, P = 0.02) (E). Cultures were grown in tryptone broth for 5 h with aeration.