TABLE 2.
Plasmids
| Plasmida | Relevant features | Source or reference |
|---|---|---|
| pDEV | colE1 lacIq Plac kan | 28 |
| pAM238 | pSC101 Plac spec | 64 |
| pDSW361 | pDSW361 is a Kanr derivative of pDSW204; pBR lacIq Ptrc99A kan | 63 |
| pJFK118EH | pMB1 lacIq Ptac kan | 42 |
| pSK12 | pAM238-Plac::mrcB | S. Kannan |
| pUB2H | pAM238-Plac::mrcB Δ(340–573)b | This work |
| pSK17 | pAM238-Plac::mrcA | S. Kannan |
| pMAJ60 | pDSW361-Ptrc99A::mrcA | This work |
| pMAJ63 | pDSW361-Ptrc99A::mrcA-lpoA | This work |
| pLpoB | pDEV-Plac::lpoB | 28 |
| pLpoB-IM | pDEV-Plac::lpoB (T62C, G36C, G65A) | This work |
| pPBP1B | pJFK118EH-Ptac::mrcB | 42 |
| pGT*TP* | pJFK118EH-Ptac::mrcB (G697C, T1528G) | 42 |
| pGT*TP | pJFK118EH-Ptac::mrcB (G697C) | 42 |
| pGTTP* | pJFK118EH-Ptac::mrcB (T1528G) | 42 |
a
Inactive domains of PBP1B are indicated with asterisks: GT*, glycosyltransferase with the E233Q mutation; TP*, transpeptidase with the S510A mutation.
b
Δ(340–573), deletion of nucleotides 340 to 573.