FIG 3.

The majority of suppressor mutations are in hspR and restore heat shock resistance. (Top) Heat shock survival assay of M. tuberculosis strains incubated for 24 h at the indicated temperature. CFU were enumerated 2 to 3 weeks later. Statistical analysis was done by nonparametric Student's t test. *, P < 0.05. Error bars represent standard deviations for three replicates. (Bottom) Immunoblots of total cell lysates of the same strains grown at 37°C using antibodies to the proteins indicated on the left. The following strains were used (from left to right, according to the strain collection number): MHD920, MHD932, MHD1160, MHD1202, MHD1203, MHD1265, and MHD1268. Genotypes are listed in Table 1.