Figure 1.

Schematic representation showing the hypoxia/reoxygenation (H/R) protocol applied to each experimental group. At normoxic conditions, siRNA-Neg (control group) and siRNA-MCU cardiomyocytes were subjected to 3 h of normothermic hypoxia (H) with an ischemic Tyrode solution (IT) followed by 3 h of reoxygenation (R). Transfected cardiomyocytes were harvested when indicated by arrows: measurements of necrosis and apoptosis by flow cytometry were performed and caspases 3 and 7 and caspases 9 and 8 activity were determined as described in Materials and Methods. Nxy, normoxy.