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. 2017 Feb 28;2017:2395138. doi: 10.1155/2017/2395138

Figure 2.

Figure 2

(a) Cell yield (×106 per g of cartilage) for incubation times of 1 h and 12 h with 750 or 3000 U/ml of collagenase with physical agitation (N = 12). (b) Cell viability (%). (c) Population doubling time (days) with initial seeding density of 5 × 103 cells/cm2. ASignificance to 1 h incubation for the same enzyme concentration and BSignificance to 750 U/ml enzyme concentration for the same incubation period (p < 0.05). (d) Evaluation of cell morphology with crystal violet (CV), hematoxylin and eosin (H&E), and fluorescent DAPI/F-Actin staining following 7 days of expansion (initial seeding density 5 × 103 cells/cm2). Scale bar: 500 µm.