Figure 2.

Local variability of the parameter CNFL. CNFL parameter local variability was calculated using published and dedicated algorithms. Heat maps (a) and density plots (b) of CNFL parameter values (in IVCM and histology images, respectively) for frames at all center positions showing local variations based on nerve fiber distribution and local fiber properties. The red lines in the plots indicate the reference value calculated for the total image areas whereas the green lines indicate the mean value of all single frames. Considerable difference in the reference and mean values was observed in IVCM mosaicking image whereas no such difference between reference and mean values for the parameter CNFL was observed in histology. Scale bar, 100 μm.