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. 2017 Feb 27;2017:3738071. doi: 10.1155/2017/3738071

Table 1.

List of sequences of forward and reverse primers.

Genes Species Forward primer Reverse primer
α-SMA Human 5′-CTATGAGGGCTATGCCTTGCC-3′ 5′-GCTCAGCAGTAGTAACGAAGGA-3′
Collagen I Human 5′-GTGCGATGACGTGATCTGTGA-3′ 5′-CGGTGGTTTCTTGGTCGG-3
Collagen III Human 5′-TTGAAGGAGGATGTTCCCATCT-3′ 5′-ACAGACACATATTTGGCATGGT-3′
K6 Human 5′-ACGGAAACTACTACGGCGAC-3′ 5′-GGCCTTCGTATCCACAGCAC-3′
K16 Human 5′-GACCGGCGGAGATGTGAAC-3′ 5′-CTGCTCGTACTGGTCACGC-3′
C-Myc Human 5′-AATAGAGCTGCTTCGCCTAGA-3′ 5′-GAGGTGGTTCATACTGAGCAAG-3′
GAPDH Human 5′-CTGGGCTACACTGAGCACC-3′ 5′-AAGTGGTCGTTGAGGGCAATG-3′
α-SMA Mouse 5′-GTCCCAGACATCAGGGAGTAA-3′ 5′-TCGGATACTTCAGCGTCAGGA-3′
Collagen I Mouse 5′-GCTCCTCTTAGGGGCCACT-3′ 5′-CCACGTCTCACCATTGGGG-3′
Collagen III Mouse 5′-CTGTAACATGGAAACTGGGGAAA-3′ 5′-CCATAGCTGAACTGAAAACCACC-3′
 GAPDH Mouse 5′-AGGTCGGTGTGAACGGATTTG-3′ 5′-TGTAGACCATGTAGTTGAGGTCA-3′

PCR amplification conditions on the Applied Biosystems 7500 Real-Time PCR System: 58°C for 5 minutes; 95°C for 2 minutes; 40 cycles of 95°C for 15 seconds and 60°C for 60 seconds.