Table 1.
Genes | Species | Forward primer | Reverse primer |
---|---|---|---|
α-SMA | Human | 5′-CTATGAGGGCTATGCCTTGCC-3′ | 5′-GCTCAGCAGTAGTAACGAAGGA-3′ |
Collagen I | Human | 5′-GTGCGATGACGTGATCTGTGA-3′ | 5′-CGGTGGTTTCTTGGTCGG-3 |
Collagen III | Human | 5′-TTGAAGGAGGATGTTCCCATCT-3′ | 5′-ACAGACACATATTTGGCATGGT-3′ |
K6 | Human | 5′-ACGGAAACTACTACGGCGAC-3′ | 5′-GGCCTTCGTATCCACAGCAC-3′ |
K16 | Human | 5′-GACCGGCGGAGATGTGAAC-3′ | 5′-CTGCTCGTACTGGTCACGC-3′ |
C-Myc | Human | 5′-AATAGAGCTGCTTCGCCTAGA-3′ | 5′-GAGGTGGTTCATACTGAGCAAG-3′ |
GAPDH | Human | 5′-CTGGGCTACACTGAGCACC-3′ | 5′-AAGTGGTCGTTGAGGGCAATG-3′ |
α-SMA | Mouse | 5′-GTCCCAGACATCAGGGAGTAA-3′ | 5′-TCGGATACTTCAGCGTCAGGA-3′ |
Collagen I | Mouse | 5′-GCTCCTCTTAGGGGCCACT-3′ | 5′-CCACGTCTCACCATTGGGG-3′ |
Collagen III | Mouse | 5′-CTGTAACATGGAAACTGGGGAAA-3′ | 5′-CCATAGCTGAACTGAAAACCACC-3′ |
GAPDH | Mouse | 5′-AGGTCGGTGTGAACGGATTTG-3′ | 5′-TGTAGACCATGTAGTTGAGGTCA-3′ |
PCR amplification conditions on the Applied Biosystems 7500 Real-Time PCR System: 58°C for 5 minutes; 95°C for 2 minutes; 40 cycles of 95°C for 15 seconds and 60°C for 60 seconds.