Skip to main content
. 2017 Feb 27;2017:5252840. doi: 10.1155/2017/5252840

Figure 3.

Figure 3

AKT inhibitors downregulate lipogenesis and inflammation in DEPTOR overexpressed cells. (a) Western blots and quantifications showed that mTORC1 activity, indicated by phosphorylations of p70S6K and 4EBP1, was downregulated by myc-DEPTOR overexpression and MK-2206 (1 μM for 4 hours, same as follows) conditions. However, mTORC2 activity (indicated by pAKT/AKT) was blocked by MK-2206 treatment in lymphocytes of PBMC culture. Results are averages of three independent experiments. Data represent mean ± SEM. Data represent mean ± SEM. ∗∗P < 0.01 and ∗∗∗P < 0.001. (b) Real-time RCR results showed that mRNA levels of lipogenesis genes, including Acl, Acc1, Fasn, and Scd1, were dramatically reduced in MK-2206 treated DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. Data represent mean ± SEM. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. (c) Real-time RCR results showed that mRNA levels of inflammation genes, including IL1-β, IL-6, and TNF-α, were dramatically reduced in MK-2206 treated DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. ∗P < 0.05 and ∗∗∗P < 0.001. (d) ELISA results showed that protein levels of TNF-α were decreased in MK-2206 treated DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. ∗P < 0.05.