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Relative abundance of Cx40 transcript in porcine follicular granulosa cells analyzed during 168 h of IVC. Porcine follicular granulosa cells were isolated from pubertal gilts and immediately used to isolate RNA, which was reverse-transcribed into cDNA. The relative abundance of Cx40 mRNA was evaluated by RT-qPCR analysis before and after each 24 h of IVC. The results are presented as the mean ± SEM with the level of significance shown as ∗∗P < 0.01.
