Figure 5. Deletion of the β₂AR gene normalizes myocyte contractile response to adrenergic stimulation after HFD feeding.

Western blots show PKA phosphorylation of PLB and TnI, and SERCA2a expression (A and B), as well as expression of cardiac adrenergic signalling regulatory proteins in the hearts of β₂AR‐KO mice fed NC and HFD (C and D). E, myocyte contractile shortening in response to β‐adrenergic stimulation (ISO, 100 nm) was examined in NC and HFD β₂AR‐KO cardiomyocytes. ^*** P < 0.001 by one‐way ANOVA followed by Tukey's post hoc test.