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. 2017 Feb 8;595(6):2065–2084. doi: 10.1113/JP273350

Table 1.

Primer sequences used in quantitative PCR

Gene Primer sequences
  • mouse GAPDH (glyceraldehyde‐3‐phosphate dehydrogenase)

  • (accession number: NM_001001303)

  • Forward primer:

  • 5′‐TTGATGGCAACAATCTCCAC‐3′

  • Reverse primer:

  • 5′‐CGTCCCGTAGACAAAATGGT‐3′

  • mouse B2M

  • (beta‐2 microglobulin)

  • (accession number: NM_009735)

  • Forward primer:

  • 5′‐TTCAGTATGTTCGGCTTCCC‐3′

  • Reverse primer:

  • 5′‐TGGTGCTTGTCTCACTGACC‐3′

  • mouse β‐ actin

  • (accession number: NM_007393)

  • Forward primer:

  • 5′‐ATGGAGGGGAATACAGCCC‐3′

  • Reverse primer:

  • 5′‐TTCTTTGCAGCTCCTTCGTT‐3′

  • Forward primer:

  • 5′‐GTGCAGATGTGGAGCGTCT‐3′

  • Reverse primer:

  • 5′‐GTTGAGGCTGGCCAAGAG‐3′

  • mouse Cx43

  • (connexin 43)

  • (accession number: X61576.1)

  • Forward primer:

  • 5′‐GTGCCGGCTTCACTTTCA‐3′

  • Reverse primer:

  • 5′‐GGAGTAGGCTTGGACCTTGTC‐3′

  • mouse PTGS2

  • (Prostaglandin‐endoperoxide synthase 2)

  • (accession number: NM_011198.3)

  • Forward primer:

  • 5′‐GGGAGTCTGGAACATTGTGAA‐3′

  • Reverse primer:

  • 5′‐GTGCACATTGTAAGTAGGTGGACT‐3′