Table 1.
Strains and plasmids used in this study.
| Strain | Description | Source |
|---|---|---|
| Natrinema sp. J7-1 | With SNJ1 proviral genome pHH205, cannot be infected by SNJ1 | [28, 34] |
| Natrinema sp. J7-F | ΔpyrF, can be infected by SNJ1 | Yuping Huang, Wuhan University, Wuhan, China |
| Escherichia coli DH5α | SupE44 ΔlacU169(φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | CCTCC |
| Escherichia coli JM110 | dam dcm supE44 hsdR17 thi leu rpsL lacY galK galT ara tonA thr tsx Δ(lac-proAB) F′(traD36 proAB+lacIq lacZ ΔM15) | CCTCC |
| Plasmid | ||
| pNBK-F | AmpR, MevR, used to clone Pfdx-pyrF resistance fragment | Yuping Huang, Wuhan University, Wuhan, China |
| pUC-M | pUC19 with SnaBI, MfeI, AgeI inserted between BamHI and Xbal | Our lab |
| pUC-M-pyrF | pUC-M with the insertion of 0.95-kb BamHI-SnaBI Pfdx-pyrF resistance fragment | This study |
| pFJ1 | pUC-M-pyrF with the insertion of the 2.8-kb SnaBI-MfeI predicted chromosomal ARS-1 | This study |
| pFJ2 | pUC-M-pyrF with the insertion of the 3.3-kb SnaBI-MfeI predicted chromosomal ARS-2 | This study |
| pFJ3 | pUC-M-pyrF with the insertion of the 3.2-kb SnaBI-MfeI predicted chromosomal r ARS-3 | This study |
| pFJ4 | pUC-M-pyrF with the insertion of the 2.2-kb SnaBI-MfeI predicted chromosomal ARS-4 | This study |
| pFJ5 | pUC-M-pyrF with the insertion of the 2.3-kb SnaBI-MfeI predicted chromosomal ARS-5 | This study |
| pFJ6 | pUC-M-pyrF with the insertion of the 2.0-kb SnaBI-MfeI predicted chromosomal ARS-6 | This study |
| pFJ7 | pUC-M-pyrF with the insertion of the 1.1-kb SnaBI-MfeI predicted chromosomal ARS-7 | This study |
| pFJ1-Apro-amyH | pFJ1 with the insertion of 1.5-kb MfeI-SphI Apro-amyH fragment containing AflII, SpeI, NotI, NheI, and NsiI | This study |
| pFJ1-M | pFJ1-Apro-amyH digested with NotI and then ligated by itself | This study |
| pFJ4-Apro-amyH | pFJ4 with the insertion of 1.5-kb MfeI-SphI Apro-amyH fragment containing AflII, SpeI, NotI, NheI, and NsiI | This study |
| pFJ4-M | pFJ4-Apro-amyH digested with NotI and then ligated by itself | This study |
| pFJ6-Apro-amyH | pFJ6 with the insertion of 1.5-kb MfeI-SphI Apro-amyH fragment containing AflII, SpeI, NotI, NheI, and NsiI | This study |
| pFJ6-M | pFJ6-Apro-amyH digested with NotI and then ligated by itself | This study |
| pFJ1-M-pro3916 | pFJ1-M with the insertion of 200-bp NsiI-SphI pro3916 | This study |
| pFJ4-M-pro3916 | pFJ4-M with the insertion of 200-bp NsiI-SphI pro3916 | This study |
| pFJ6-M-pro3916 | pFJ6-M with the insertion of 200-bp NsiI-SphI pro3916 | This study |