Table 2.
Primers used in this study.
| Primers | 5′-3′ sequence | Restriction sites |
|---|---|---|
| Pfdx-pyrF-F | AATGGATCCATCTCGGCTTATTCTTTTGATT | BamHI |
| Pfdx-pyrF-R | TAATACGTATTATTCTCGATACTGATTGAGTCGCTTC | SnaBI |
| PARS-1-fwd | AATTACGTACGCCCCCGGTGCCTCCTCTCGGA | SnaBI |
| PARS-1-rev | ATTCAATTGACTCGCCGCCGACTACCTCCCCGTCG | MfeI |
| PARS-2-fwd | AATTACGTATAGCCCGGGAAATACTATCTTTGAGTTCT | SnaBI |
| PARS-2-rev | ATTCAATTGGATCGACGCTGGGATATGAAAAGC | MfeI |
| PARS-3-fwd | AATTACGTAAACGGCTTTCGGATCGAAAGCAGC | SnaBI |
| PARS-3-rwd | ATTCAATTGTTCGGTCTGCGGTCCCCATTTCC | MfeI |
| PARS-4-fwd | AATTACGTAGACACACACCACTGTTGCAAGTGAAG | SnaBI |
| PARS-4-rev | ATTCAATTGGTGGCCGCACAAGATCGA | MfeI |
| PARS-5-fwd | AATTACGTACGATCGTGCCGACGTTACCCGGT | SnaBI |
| PARS-5-rev | ATTCAATTGCGATCCCGAAGACGACCGCGT | MfeI |
| PARS-6-fwd | AATTACGTAGGAGACGGTCAGAGTTACTGGTCAGT | SnaBI |
| PARS-6-rev | TAACAATTGCAAGGGTTCGTCTGAAACCGTGT | MfeI |
| PARS-7-fwd | ATATACGTAAGGCTGACTGTATGCGAGT | SnaBI |
| PARS-7-rev | ATACAATTGGCACGACAGTAACAGT | MfeI |
| 3916pro(R)-F | AATATGCATGAGTAAAGTTCGTGTTTCCTTGATTA | NsiI |
| 3916pro(R)-R | ATAGCATGCCGGACAAGACGCCCATTTG | SnaBI |
| Apro-AmyH-M-F | AATCAATTGCTTAAGACTAGTGCGGCCGCGGGAGCCGGAAACGCGGTAGAGATA | MfeI, AgeI, SpeI, NotI |
| Apro-AmyH-M-R | AAGCATGCTATGCATAGCTAGCGCGGCCGCAAGGTAGTGGAAAGCGAGCCAGCGC | NotI, NsiI, NdeI, SphI |
| pyrF test-F | CGATCACCGTCAACCCCTACATGG | / |
| pyrF test-R | TACTGATTGAGTCGCTTCTTCAGTCGTTT | / |
| MevR test-F | TCGCCTCCCTCGAAGTCGGCACCGT | / |
| MevR test-R | GAACAACGGCGAAGAAAAGGCAGTCCA | / |