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. 2017 Mar 6;216(3):657–674. doi: 10.1083/jcb.201507113

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© 2017 Saunders et al.

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Figure 2. — The redox-regulated ATPase activity of TA is required for rearward nuclear positioning and centrosome orientation. (A) Diagram indicating the location of the designated mutations in the TA constructs used in this figure. (B) Representative epifluorescence images of centrosome orientation in wound-edge NIH3T3 fibroblasts expressing the indicated construct (arrowheads and insets). (C) Centrosome orientation in the cells described in B. (D) Mean centrosome and nucleus positions in the cells described in B. n ≥ 69. (E) Representative epifluorescence images of centrosome orientation in Tor1A^−/− MEFs expressing the indicated SS-EGFP-TA^X constructs (arrowheads and inserts). Asterisks indicate oriented centrosomes. (F) Centrosome orientation in the cells described in E. (C and F) Dashed yellow lines denote random centrosome orientation. (G) Mean centrosome and nucleus positions in the cells described in E. n ≥ 115. Bars, 10 µm. Error bars show SEM. **, P < 0.01; ****, P < 0.0001; ns, not significant.