Figure 4.

Peroxisome-targeted BAK abrogates peroxisome biogenesis. (A) Schematic presentation of peroxisome-targeting BAK-P26. The asterisk designates an L78A mutation in the BH3 domain of BAK. Solid bars indicate a transmembrane segment (TM). (B and C) Flag–BAK-P26 and Flag–BAK-P26–L78A were expressed in CHO-K1 (B) and HeLa (C) cells. The cells were immunostained with antibodies to catalase, Flag, and Pex14p. Merged views of a–c and e–g are shown in d and h, respectively, where three fluorescent colors, red (R), green (G), and blue (B), were merged. Bars, 10 µm.