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. 2017 Mar 6;216(3):709–722. doi: 10.1083/jcb.201605002

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© 2017 Hosoi et al.

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Figure 7. — Overexpression of BH3-only proteins PUMA and BIM, but not of BAD, disrupts peroxisomes in a BAK-dependent manner. (A and B) Control and BAK-KD#2 cells (see Fig. 6, B–D) were transfected with Flag-PUMA, Flag-BIM, and Flag-BAD in the presence of a caspase inhibitor, Q-VD-Oph. At 48 h after transfection, cells were immunostained with antibodies to Flag, Pex14p, and catalase (A) or cytochrome c (B). Asterisks indicate respective transfectants. Merged views of a–c, e–g, i–k, m–o, q–s, and u–w are shown in d, h, l, p, t, and x, respectively. Bars, 10 µm. Bottom graphs show percentages of the cells showing released catalase (A) or cytochrome c (B) in cells expressing Flag-tagged proteins. Data represent means ± SD.