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. 2017 Feb 20;36(6):783–796. doi: 10.15252/embj.201593378

Figure EV2. KJE acts prior to disaggregation of IbpAB–MDH assemblies.

Figure EV2

An order of addition experiment. MDH (2 μM) was denatured in the presence of IbpA (3 μM) and IbpB (7 μM) at 47°C for 30 min. IbpAB–MDH assemblies were diluted fourfold and first incubated with the components listed in the legend and then complemented with the missing components of the functional ClpB‐KJE bichaperone system. The DnaK, DnaJ, GrpE, ClpB concentrations used were as follows: 1, 0.3, 0.3 and 1.5 μM. Data are the mean ± SD of three independent experiments.