Figure 5. sHsps spontaneously dissociate from and re‐associate with sHsp–substrate assemblies.

Surface‐exposed IbpAB are dynamically binding and dissociating from the assemblies. Purified IbpAB–luciferase assemblies (100 nM; calculated for luciferase monomer; approximate stoichiometry 1:0.8:1) were incubated with the indicated peptides (1 mM each) for 60 min and subjected to glycerol gradient sedimentation. Fractions were collected from the top of the gradient, and luciferase and IbpA were visualized by Western blot following SDS–PAGE. Source data are available online for this figure.